Direct PCR Featured

Finnzymes’ novel approach, Direct PCR, saves you time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Finnzymes' Phusion® High-Fidelity DNA Polymerases and Phire® Hot Start DNA Polymerase. These unique fusion enzymes have been engineered to incorporate a double-stranded DNA binding domain that gives them extraordinary properties. Phusion and Phire DNA Polymerases are exceptionally tolerant of many PCR inhibitors present in various sample material. This allows robust amplification in conditions where conventional DNA polymerases, like Taq or Pfu, are completely inhibited.

PCR directly from blood
Phusion® Blood Direct PCR Kit is designed for amplification of DNA directly from whole blood.
See the product page for examples and additional information. Application note: Direct PCR from blood preserved on Whatman FTA® and 903® Cards Application protocol: PCR-RFLP directly from blood using Phusion® Blood Direct PCR Kit

PCR directly from plants

NEW! Phire® Plant Direct PCR Kit includes a complete set of optimized reagents, sampling tools and control primers for convenient amplification of plant DNA.

See the product page for examples and additional information. Which plant materials work with Phire Plant Direct PCR Kit? (updated June 2009)

PCR directly from mouse ear and tail tissue
Application note: Direct PCR from mouse tissue using Phire™ Hot Start DNA Polymerase Updated protocol! (May 2009)Direct PCR protocol for mouse tissue
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  • Direct PCR from mouse ear and tail tissue samples

PCR directly from formalin-fixed paraffin-embedded (FFPE) tissues
Application note: PCR from FFPE tissues without DNA extraction using Phusion® DNA Polymerase
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  • Direct PCR from prostate tissue section
  • Comparing performance of Phusion® DNA Polymerase to Taq DNA polymerase
  • Comparing the simple disruption protocol to a commercial DNA extraction kit

PCR directly from bird feathers
Application protocol: Direct PCR for bird feathers
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  • Direct PCR from barb, rachis and quill-end
  • Comparing performance of Phire® DNA Polymerase to Taq DNA polymerase

PCR directly from muscle tissue
Application protocol: Direct PCR for muscle tissue
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  • Direct PCR from muscle tissue of different animals
  • Comparing performance of Phire® DNA Polymerase to Taq DNA polymerase

Note that PCR from unpurified starting material may require more optimization than standard PCR reactions from purified DNA. Also, the Direct PCR approach may not be suitable for all applications. So, please read the protocols carefully when planning your Direct PCR experiments. If you have questions or comments, please don't hesitate to contact us or your local distributor.

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