Product Portfolio
Automated High Throughput Systems
The adaptation of well established Invitek HTS kits using the Invisorb, InviTrap and MSB technology to common liquid handling workstations, e.g. the Eppendorf epMotion 5075 platforms, the X-tractor Gene™ (now: QIAGEN QIAxtractor) or the Tecan station Freedom Evo 200 etc., allows a simple and convenient walk-away or semi-automated isolation of DNA fragments and PCR products, genomic DNA, mitochondrial DNA, bacterial DNA, viral DNA and RNA as well as total RNA from different starting materials. Procedures previously carried out manually can now be easily and quickly performed.
Advantages:
- high-speed fully automated system
- high throughput – up to 96 samples per run
- cross contamination free process
- purification of high-quality, ready-to-use nucleic acids
- ready-made and customized purification protocols
- high binding capacity and recovery values of membranes
Kit Overview
| Sample Source | Recommended Kit | Sample Volume | Yield |
| Plasmid DNA | |||
| Bacterial suspension, bacteria pellets | Invisorb Plasmid HTS 96 Kit/ R | 0.5 - 1.0 ml | 10 μg |
| DNA Fragment Purification | |||
| PCR products, ligation reaction mixture, enzyme digestion mixture, cDNA synthesis mixture | MSB HTS PCRapace/ R | up to 100 µl | 80 – 95 % recovery |
| Genomic DNA Purification | |||
| Whole human or animal blood, buffy coat | Invisorb Blood Mini HTS 96 Kit/ R Invisorb Blood Midi HTS 96 Kit/ R |
up to 200 µl up to 2 ml |
2 – 10 µg 10 – 30 µg |
| Human or animal tissue, rodent tails, paraffin embedded tissue, mammalian cells | Invisorb DNA Tissue HTS 96 Kit/ R | up to 20 mg 0.5 – 0.8 cm rodent tails up to106cells |
10 – 25 µg |
| Human and animal cells | Invisorb DNA Cell HTS 96 Kit/ R | 5 x 105 cells | 10 – 20 µg |
| Saliva, swabs | PSP SalivaGene DNA HTS 96 Kit/ R | 500 µl | up to 10 µg |
| Forensic material | Invisorb Forensic HTS 96 Kit/ R | depending on type and amount of starting material | depending on type and amount of starting material |
| Nucleic Acid Purification from Pathogens | |||
| Bacterial DNA | |||
| Serum, plasma, whole blood, cell-free body fluids, swabs, bacterial cultures, tissue material | Invisorb Universal Bacteria DNA HTS 96 Kit/ R | up to 100 µl5 – 10 mg tissue material | depending on starting material |
| Saliva, swabs | PSP SalivaGene DNA HTS 96 Kit/ R | 500 µl | up to 10 µg |
| Viral DNA | |||
| Plasma, serum, cell-free body fluids, rinse liquid from swabs, stool samples | Invisorb Virus DNA HTS 96 Kit/ R | up to 200 µl | depending on starting material |
| Viral RNA | |||
| Plasma, serum, whole blood, cell-free body fluids, rinse liquid from swabs | Invisorb Virus RNA HTS 96 Kit/ R | up to 200 µl | depending on starting material |
| Total RNA isolation | |||
| Human or animal cells | InviTrap RNA Cell HTS 96 Kit/ R InviTrap Universal RNA HTS 96 Kit/ R |
5 x 105 cells 1 x 107 cells |
10 – 20 µg up to 100 µg |
| Human or animal tissue | InviTrap Universal RNA HTS 96 Kit/ R | up to 10 mg | up to 80 µg |
| Whole human or animal blood | InviTrap Universal RNA HTS 96 Kit/ R | up to 1 ml | up to 1 – 5 µg total RNA/ml |
Automation resources - implementation on robotic workstations
Invitek offers a variety of kits for full automation based on different technologies (membrane, magnetic beads). Long years of experience in kit development and optimization in combination with a thorough understanding of requirements, chances and limitations in automation have resulted in highly optimized solutions for automated needs. A team of experienced Invitek application specialists will perform the integration of the HTS 96 kit on the customer’s robotic workstation, either.
- in collaboration with the customers themselves or
- with specialists from the producer of the laboratory workstations.
Furthermore, the team will develop solutions for nucleic acid purification on customer specific demands (on request).
PLEASE NOTE:
The RNA kits with the trademark Invisorb have been renamed as InviTrap to communicate the selective DNA removal for enhanced RT-PCR assays