Product Portfolio
High Throughput Systems
Invitek offers a broad range of products for high throughput applications, which are developed for the highly efficient purification of plasmid DNA, PCR products, genomic DNA, genomic DNA from forensic and human-identity samples, bacterial DNA, viral DNA and RNA and total RNA from a wide range of starting materials.
Advantages:
- use patented technology
- manual processing under vacuum or by centrifugation possible
- suitable for common liquid handling instruments
- easy implementation on robotic workstations
- consistently yield nucleic acids of the highest quality
- use very fast and simple procedures
- no cross-contamination
- the MSB HTS kit simply involves two steps: binding and elution
- the InviTrap HTS systems for RNA isolation work without DNase digestion
- resulting nucleic acids provide accurate, reliable gene expression data
Kit Overview
| Sample Source | Recommended Kit | Sample Volume / well | Yield / well |
| Plasmid DNA Purification | |||
| Bacterial suspension, bacteria pellets | Invisorb Plasmid HTS 96 Kit | 0.5 - 2.0 ml | 10 - 25 μg |
| DNA Fragment Purification | |||
| PCR products, ligation reaction mixture, enzyme digestion mixture, cDNA synthesis mixture | MSB HTS PCRapace | up to 100 µl | 80 – 95 % recovery |
| Genomic DNA Purification | |||
| Whole human or animal blood, buffy coat, bone marrow | Invisorb Blood Mini HTS 96 Kit Invisorb Blood Midi HTS 96 Kit |
depending on type and amount of starting material | depending on type and amount of starting material |
| Fresh or frozen tissue, rodent tail, | Invisorb DNA Tissue HTS 96 Kit | up to 20 mg tissue, 1.0 cm mouse tail, 0.6 cm rat tail |
up to 30 μg |
| Human and animal cells | Invisorb DNA Cell HTS 96 Kit Invisorb DNA Tissue HTS 96 Kit |
5 x 105 - 5 x 106 cells 1 x 106 cells |
up to 20 μg |
| Saliva, swabs | PSP SalivaGene DNA HTS 96 Kit | 500 µl | up to 10 µg |
| Forensic material | Invisorb Forensic HTS 96 Kit | depending on type and amount of starting material | depending on type and amount of starting material |
| Plant material | Invisorb DNA Plant HTS 96 Kit | up to 50 mg | up to 10 µg |
| Nucleic Acid Purification from Pathogens | |||
| Bacterial DNA | |||
| Serum, plasma, whole blood, cell-free body fluids, bacterial cultures, food, tissue | Invisorb Universal Bacteria DNA HTS 96 Kit | up to 100 µl | depending on starting material |
| Saliva, swabs | PSP SalivaGene DNA HTS 96 Kit | 500 µl | up to 10 µg |
| Viral DNA | |||
| Plasma, serum, whole blood,cell-free body fluids, rinse liquid from swabs, | Invisorb Virus DNA HTS 96 Kit | up to 200 µl | depending on starting material |
| Viral RNA | |||
| Plasma, serum, cell-free body fluids, rinse liquid from swabs, stool samples | Invisorb Virus RNA HTS 96 Kit | up to 200 µl | depending on starting material |
| Total RNA isolation | |||
| Whole human or animal blood | InviTrap RNA Universal HTS 96 Kit | up to 1.0 ml | up to 1 – 5 µg/ ml |
| Human or animal cells | InviTrap RNA Cell HTS 96 Kit InviTrap RNA Universal HTS 96 Kit |
5 x 105 cells up to 1 x 107 cells |
10 - 15 µg up to 100 µg |
| Human or animal tissue | InviTrap RNA Universal HTS 96 Kit | up to 10 mg | up to 80 µg |
| Plant material | InviTrap RNA Plant HTS 96 Kit | up to 50 mg | up to 30 µg |
General procedure for use of the HTS kits
Following efficient lysis and a precleaning step, if necessary, the nucleic acids bind to specific filter membranes. Very efficient washing steps remove all impurities and ultra pure nucleic acids are eluted in a low-salt buffer. Invitek’s sample preparation technologies are patented and yield nucleic acids of the highest quality, ready to use in any downstream applications.
The HTS PCR cleanup system uses the patented MSB technology. After adjustment of binding conditions, the DNA fragments bind to filter membranes. No washing steps are required and ultra pure PCR fragments of the highest quality, ready to use in any downstream application, are eluted in a low-salt buffer or water.
Centrifuge or vacuum
All Invisorb HTS kits and InviTrap and the MSB HTS kit are optimized for use in a centrifuge with deep buckets, realizing very short processing times and the highest yields. Samples can be processed within 15 min - 1.5 hours using these procedures (less than 10 sec - 1 minute per preparation). The kits are also suitable and optimized for vacuum driven processing and can be deployed on manifolds, e.g. the Invisorb 96 Vacuum Manifold, or on common robotic workstations for completely automated purification procedures.
PLEASE NOTE:
The RNA kits with the trademark Invisorb have been renamed as InviTrap to communicate the selective DNA removal for enhanced RT-PCR assays these procedures