Automation from barcode reading, sampling from primary tube, nucleic acid purification, master mix preparation, reaction (PCR) setup, and reaction (PCR) with throughput 1 to 48

Product Description

LabTurbo 48S system is a multifunctional DNA/RNA workstation with the functions of barcode tracking, sampling from primary tubes, nucleic acid purification, and multiplex/array PCR setup. Samples can be loaded from primary tubes with the barcode information recorded. By the membrane column chemistry and optimized protocol, the system enables nucleic acid purification from various biological samples with the high yield, purity, and sensitivity and without cross-contamination. Subsequent multiplex/array PCR setup and reaction (PCR) can be done without manual input. LabTurbo 48S standardizes procedures from nucleic acid extraction to PCR, saves the labor/cost on routine tasks, and minimizes human errors as well.

Features

• General —

♦ Compact, all-in-one design

♦ Low-to-medium throughput

♦ Dual (6 + 1) channel pipette for different working volumes of DNA/RNA purification and PCR setup

♦ High pipetting precision, CV < 5%

♦ Safety door

♦ User-friendly interface

♦ Built-in setup checkilist and reagent calculator

♦ Data filing as Excel files

♦ UV sterilization

♦ Straightforward installation and maintenance

♦ HEPA option

♦ Tested by TÜV, and has CE marking; Compliance with ISO13485

• Barcode Tracking —
♦ Barcode reading of primary sample tubes and elution tubes
♦ Cross-matching function
♦ Automatical input of consecutive barcode number

• Sampling from Primary Tube —
♦ Ultrasonic sensor measures the level of input sample
♦ Bottom detection enables sampling from small-sized primary tubes
♦ Multi-syringe pipetting
♦ Customized primary tube rack

• DNA/RNA Purification —
♦ 80 min for 48 preps
♦ Central processing unit (CPU) design prevents cross-contamination and faciliates the workflow
♦ Ultrasonic fluid sensors detects the liquid level in spin columns and in buffer tanks
♦ Preinstalled, optimized protocol
♦ Reagent loading checklist
♦ Thermo-lysis
♦ Membrane column binding by vaccum force
♦ Impurity wash-away strategy
♦ Column clean-and-dry technique eliminates residual organic solvent and increases purity of products
♦ Thermo-elution in the isolated elution block
♦ OD A₂₆₀/A₂₈₀ 1.7 - 1.9 for DNA; 1.9 - 2.1 for RNA
♦ Cross - contamination tolerance higher than 10¹⁰ copies of virus per ml

• Reaction (PCR) Setup —
♦ Master mix (4) preparation from (PCR) component (12)
♦ PCR setup including standard, positive control, negative control templates, and HSC (human specimen control)
♦ PCR preparation in 8-tube, 12-tube strips, or 96-well PCR plate
♦ Cooling system for proteinase K, PCR component, and PCR sample mix
♦ Mineral oil is available
♦ Free to selelct any starting position for templates and PCR samples
♦ All recipes of PCR setup can be recorded as Excel file

• PCR —
♦ Flexibility to setup the time, temperature, and cycle of the thermo-profile
♦ Dust-prevented slide for PCR sample mix

Specification

Throughput	1-48 extractions per run in 80 min
Sample volume	300 µl (10- 900 µl)
Function	Barcode tracking Sampling from primary tube Nucleic acid purification Master mix preparation PCR setup General liquid handling
Working unit	Dual (6+1) channel pipette Volume range: 5 — 1000 µl (CV < 5%) 96-well tip rack (4) CPU design (6x8) Thermal sample rack (48) Dry slot (8) Binding-washing vacuum manifold (48) Ultrasonic fluid sensor (1) Elution vacuum manifold (48) Prewarmed elution buffer rack (6) Tip re-use rack and tip disposal (1) Reagent tank (4) Enzyme rack (6) Mineral oil rack (6) PCR master mix (4) and component (12) racks (cooling: 4-8°C) 96-well thermocycler plate for PCR Primary tube rack (optional) Pause door (1) UV light (1)
Software	Cross-matching of barcodes of primary and tubes Preinstalled, certified protocol Graphical user interface Quick button for start Worktable setup checklist Built-in reagent calculator Pause function Timer PCR setup table Thermo-profile graph Data conversion to Excel file Remote control
Isolation kit	LabTurbo Genomic DNA Mini Kit (500) LabTurbo Total RNA Mini Kit (500) LabTurbo Virus Mini Kit (500)
Vacuum station	Pump (1) Air flow: 130 L / min Waste Bottle (4) Volume: 5 L
Computer System	Shuttle All-in-one PC Windows XP
Dimensions	86 cm (W) x 79 cm (D) x 98 cm (H) 33.9" (W) x 31.1" (D) x 38.6" (H)
Weight	150 kg ( 331 lb)
Processing temperature and humidity	5-40°C and 10-90% relative, non-condensing
Power requirements	110V/220V AC
Regulatory	In compliance with CE standards