With Lonza's family of transfection tools each transfection challenge turns into a breeze. Whether you transfect standard cell lines, hard-to-transfect cell lines, primary cells or stem cells, Lonza offers a product perfectly suited to your needs.

Experience it yourself and:

• Learn more about the new 4D-Nucleofector
• Request general information about Lonza's Nucleofector Technology
• Have a look at our Nucleofection Videos
• See how transfection of cells in adherence works

Nucleofector Technology Platforms

Developed in 1998, the Amaxa Nucleofector Technology has revolutionized transfection. Since then Nucleofection has evolved through constant innovation. In 2010 Lonza's Nucleofector Portfolio comprises:

• The new 4D-Nucleofector System is the most advanced platform offering maximum flexibility in regards to cell numbers and cell status. For the first time this system enables Nucleofection of cells in adherence.
• The 96-well Shuttle Module is an add-on device compatible with the 4D-Nucleofector System. Connected to the 4D-Nucleofector System it enables higher throughput and convenient optimization.
• The new HT Nucleofector System represents an independent High Throughput system supporting up to 384 Nucleofection Reactions in parallel.
• The Nucleofector II Device. Based on the original Nucleofector device, this basic instrument enables transfection of difficult-to-transfect cells in a single cuvette. Lacking the flexibility of the 4D-Nucleofector the Nucleofector II represents the entry level model of Lonza's Nucleofection Portfolio

Transfection Reagents

Lonza's individual transfection reagents are optimized for e.g.:

• Cost effective transfection of standard cell lines
• Delivery of various substrates
• The use with Clonetics and Poietics Primary Human Cells and media

Transfection Services

Access our transfection expertise to achieve high transfection efficiencies and viabilities in almost every cell type with Lonza as your preferred supplier. Receive validated stable clones quickly. Let us optimize your Nucleofection Protocols for your cells.

Amaxa Vectors

The pmaxFP Vectors express novel green (maxFP-Green), yellow (maxFP-Yellow and maxFP-Yellow-m) and red (maxFP-Red) fluorescent proteins. These pmaxFP Vectors can be used to:

• Monitor gene expression and protein localization in living mammalian cells
• Generate green, yellow or red fluorescent C- and N-terminal fusion proteins
• Study gene-regulation by using promoter-less (PRL) versions

The pmaxCloning™ Vector promotes strong, constitutive expression of cloned DNA inserts in mammalian cells.

Amaxa Transfection Control Kits

Lonza's different transfection control kits are developed to assist in setting up your peptide transfection or to establish your siRNA experiments. Check out the Amaxa Peptide Transfection Control Kit and the siRNA Test Kit.